SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-11 18:32:56

description

unknown

similar to hydroxypyruvate reductase

locus

BSU34680

BSU_34680

geneLength

975

978

outlinks

bsu

BSU34680

BSU_34680

The protein

Catalyzed reaction/ biological activity

D-gluconate + NADP+ = 2-dehydro-D-gluconate + NADPH (according to Swiss-Prot)

D-gluconate + NADP+ --> 2-dehydro-D-gluconate + H+ + NADPH (according to UniProt)

The protein

Protein family

D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

D-isomer specific 2-hydroxyacid dehydrogenase family (with [[protein|YoaD]] and [[protein|SerA]], according to UniProt)

Biological materials

Mutant

MGNA-B633 (yvcT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1632 NBRP B. subtilis, Japan]

MGNA-B633 (yvcT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1632 NBRP B. subtilis, Japan]

BKE34680 (Δ[[gene|yvcT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC, downstream forward: _UP4_TAAATCAAAAATCCGGTCTG

BKK34680 (Δ[[gene|yvcT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGCTCCCCCTTTATGGC, downstream forward: _UP4_TAAATCAAAAATCCGGTCTG

The protein

Paralogous protein(s)

[[this]]

The protein

Structure

[PDB|5AOV] (from Pyrococcus furiosus, 46% identity) [pubmed|26865263]

References

Research papers

26865263